ABSTRACT
The present study was done to investigate the possible effect of Nigella sativa oil [NSO] on cold restraint [CR] and indomethacin [I] induced gastric ulcer in albino rats. Thirty-six adult male albino rats were used in this study; they were divided into six equal groups. Pyloric ligation was done in all rats to collect gastric juice. Gastric ulcer was induced by CR and I treatment. Oral treatment with NSO daily for two weeks was given to control [non-stressed] and to CR and I treated rats before ulcer induction. Gastric ulcer scores, gastric juice analysis for acidity, proteolytic activity and mucin, gastric mucosa1 histamine, glutathione [GSH], prostaglandin E2 [PGE2] and lipid peroxides as well as serum total antioxidant status [TAS] were determined and the mean levels were compared with control non treated parameters. It can be concluded that NSO imparted a protective action against CR- and I-induced ulcer in rats and can be recommended for prophylaxis and treatment of gastric mucosa1 injury